Contribute Seeds to the Project Baseline collection!

We request that investigators who receive seeds from the Baseline collection contribute refreshed seeds back to the collection when possible. We expect that seeds obtained from the collection may be put through a refresher generation, as advised for resurrection studies (Franks et al. 2018). In this refresher generation, plants derived from the collected ancestral seeds are grown under common conditions in growth chambers, greenhouses, field sites or other facilities and then crossed naturally or by hand within the ancestral group. If there are excess seeds that are not needed for the planned experiments from the refresher generation, they can be returned to the Baseline collection to maintain this collection and make seeds available for later studies. The seeds should ideally be collected by maternal line and with an equal contribution of seeds from each maternal plant, so that the refreshed seeds contain an unbiased representation of the ancestral gene pool, with the plants not undergoing selection during the refresher generation. The seeds should be collected, stored and shipped according to Baseline protocols. The investigators should communicate with both the Baseline advisory panel and USDA/NCGRP to determine the details.

Franks, S. J., E. Hamann, and A. E. Weis. 2018. Using the resurrection approach to understand contemporary evolution in changing environments. Evolutionary Applications 11:17-28.

Caption: A flowchart of example procedures for resurrection studies comparing ancestors and descendants to study evolution, including recommendations for best practices. Reciprocal transplantation means planting ancestors and descendants under conditions meant to approximate the conditions experienced by ancestors and descendants. The dashed line between "T2: descendant lines" and "Propagule storage" indicates that these propagules may often only be briefly stored, whereas ancestral lines are generally stored long term. (Figure from Franks et al. 2018)